detection of brucella by peripheral blood pcr and comparison with culture and serological methods in suspected cases

background: brucellosis is a zoonotic disease and its symptoms and appearances are not exclusive in human and its tradi­tional diagnosis is based on blood culture and serological methods. for more sensitive and specific detection, the pcr method is recommended. methods: one hundred four blood samples were gathered from suspicious patients with brucellosis different ages form ker­manshah, mazandaran, khorassan and hormozgan provinces, and were examined by rose bengal, anti globulin, culture and pcr methods. results: seventy three samples were positive by pcr method, 15 samples were positive by cultured method and 84 sam­ples were positive by serological methods. conclusion:  pcr method is sensitive and specific for diagnosis of brucella from peripheral blood in suspected cases.